Question: What are the main chemicals you use when you are experimenting?

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  1. I’m not currently using any chemicals. And in the past, I used so many for my experiments that I’m not sure I could list “main” ones. We went through a lot of alcohol for cleaning the benches and equipment though!

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  2. I’m guessing your after specific nasty stuff that I use bobsagget. lol
    On a day to day basis I use known carninogens and equally bad acids and organic pollutants. I won’t go into detail because I don’t think it would be of any value. What’s important is that everyone who uses these agents respects the danger behind using them, and understands why they are used. Equally important is how they should be discarded as waste.
    There are literally 100 or more ways you can die in a scientific lab. Most if not all would be as a result of you doing the wrong thing.
    Hope that helps.

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  3. Hi bobsagget

    Thanks for the conversation earlier and the interesting question!.

    In my experiments I use a number of different chemicals, mostly salts dissolved in water (to create an environment that helps keep tissue alive once removed from the experimental animal) – we call this physiological saline.

    Sometimes in the experiments I perform I may use toxins which can prevent the effect of neurons, or cause the intestine to contract violently to study the effect of toxin induced diarrhea.

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  4. I use a lot of the same types of things that you need to stay alive.
    Because i grow things in incubators (little ovens at body temperature) i need to feed them with things like glucose, and fats and amino acids that they need for survival, much like what you feed yourself with.

    I also use lots of fluorescent markers to look at different things inside the cell!

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  5. I have used a silver solution to stain neurons in thin sections of mouse brain. The neurons in the memory region of the brain, the hippocampus, are very complicated and their branches look like dense tree tops. You can search “golgi stain” in google images and have a look at similar pictures to what I see when I look down the microscope.

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